NovaSeq Data Access/Analysis

Information for Illumina NovaSeq X Plus sequencing data:

RCC Account:

In order to access the sequencing data, generate an account (guest account if outside FSU) with the FSU Research Computing Center (RCC) .

Demultiplexing:

If sequencing samples are pooled and demultiplexing is needed, the user should provide an index sheet to TSL ( yanming.yang@med.fsu.edu ; cynthia.vied@med.fsu.edu ).  The index sheet should be generated using the Illumina Experiment Manager software for demultiplexing compatibility.

Data Access:

Data Transfer:

FSU RCC preferred approach for data transfer is via Globus .

Please use the following information on the Globus transfer page:
             Endpoint:  fsurcc#GPFS#research
Path to data: /medicine/sequencer/CORRESPONDING_PATH_PROVIDED_BY_EMAIL

Analysis Software:

The following software packages and others are available for data analysis at the  RCC : FastQC, Samtools, Biopython, Abyss, Bowtie/Bowtie2, BWA, Star aligner, Tophat, Cufflinks, R packages, HTSeq, Trimmomatic.