Resources

Applications:

Cell marker profiling: With the advanced full spectrum profiling (FSP), up to 40 colors can be detected simultaneously.
Cell Sorting (FACS): Isolating specific populations (e.g., stem cells) for downstream culture or single-cell sequencing.
Cell Cycle & DNA Analysis: Measuring G ₁ , S, and G ₂ /M phases using dyes like Propidium Iodide or DAPI.
Apoptosis Assays: Distinguishing between programmed cell death and necrosis using Annexin V and 7-AAD.
Subcellular/colocalization Assay (ISX only): Cell images acquired by microscope from the flow cell were used for spatial information analysis.
Cytokine Profiling: Beads based ELISA enable profiling up to 14 soluble cytokines from 50 µl sample. BioLegend
Metabolic Profiling: Measuring protein synthesis as a proxy for metabolic state (glycolysis vs. oxidative phosphorylation) at the single-cell level. SCENITH
Extracellular Vesicle (EV) Analysis: Characterizing exosomes and microvesicles (100-1000 nm) for liquid biopsies. MIFlowCyt-EV
Phosflow: Measuring intracellular signaling by staining for phosphorylated proteins (e.g., p-STAT, p-ERK). BD
And more…

Training and Protocols:

Instrument User Manual:

Office Contacts

For more information, contact:
Li Sun
Director
Phone: 850-368-0169

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